Pastors who have served at Ebenezer are listed below.
| 1888 – 1890 | J.S. Bartan |
| 1891 | E.H. Lamar |
| 1892 | W.L. Orem |
| 1893 | E.C. Gallaher |
| 1894 – 1896 | A.F. Campbell |
| 1897 – 1898 | Thomas E. Capes |
| 1899 | Charles E. Dudrear |
| 1900 | Charles E. Dudrear and S.F. Jones |
| 1901 | Charles E. Dudrear and J.E. Metee |
| 1902 | Charles E. Dudrear and U.S. Wright |
| 1903 | Fletcher Watson and A.B. Wood |
| 1904 | Fletcher Watson and Wilson T. Jarboe |
| 1905 | George C. Harriss and Wilson T. Jarboe |
| 1906 – 1907 | George C. Harriss and W.F. Carey |
| 1908 | Charles Taylor and I. Tozer |
| 1909 | Robert E. Benson |
| 1910 – 1913 | H.C. Hall |
| 1914 – 1916 | C.H. Corkran |
| 1917 – 1919 | C.H. Wagner |
| 1920 – 1921 | Raymond L. Mowbray |
| 1922 – 1924 | J.W. Briscoe |
| 1925 | G.E.M. Justice |
| 1926 – 1928 | Lloyd Davis |
| 1929 | J.E. Reamy |
| 1920 – 1932 | Melvin Baker |
| 1933 – 1935 | Howard Amoss |
| 1936 – 1938 | Robert L. Bartlett |
| 1939 – 1941 | W. DeWitt Dickey |
| 1942 – 1943 | J. Howard Link |
| 1944 | E. Kyle Sawyers |
| 1945 | H.C. Shaffer |
| 1946 | Winfield Hall |
| 1947 – 1948 | Howard Spitnale |
| 1949 – 1951 | Elbert Perkins |
| 1952 – 1954 | E. Kyle Sawyers |
| 1955 – 1956 | Richard D. Thompson |
| 1956 – 1959 | Hoyt A. Dudley, Jr. |
| 1960 – 1962 | Charles G. Muradian and Leroy Moreland |
| 1962 | Karl Swenson |
| 1963 – 1966 | Harry Ledgard |
| 1967 – 1981 | Glenn H. Hickman |
| 1981 – 1990 | Richard G. Gray, Jr. |
| 1990 – 1996 | Richard Simpson |
| 1996 – 2006 | Gayle Annis-Forder |
| 2006 – Present | Judith A. Emerson |